- ￥5590
- Novus Biologicals
- NBP2-34720-0.1 mg
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Mouse Monoclonal TYRP1 Antibody (TA99) [Azide Free]
- 抗体英文名：
Mouse Monoclonal TYRP1 Antibody (TA99) [Azide Free]
- 克隆性：
多克隆
- 保存条件：
低温
- 规格：
0.1 mg
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
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