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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mouse Monoclonal Fatty Acid Synthase Antibody (497CT15.2.5)
- 抗体英文名:
Mouse Monoclonal Fatty Acid Synthase Antibody (497CT15.2.5)
- 克隆性:
多克隆
- 保存条件:
低温
- 规格:
0.4 ml
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文献和实验Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
The production of monoclonal antibody by hybridoma
antibody of predefined specificity.1975. Nature 256: 495-497. 2) Lane, R.D. A short duration polyethylene glycol fusion technique for increasing production of monoclonal antibody-secreting hybridomas. 1985. J. Immunol. Meth
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