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- Novus Biologicals
- NBP2-07281
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
TBC1D5 Overexpression Lysate (Native) - (Dry Ice)
- 抗体英文名：
TBC1D5 Overexpression Lysate (Native) - (Dry Ice)
- 克隆性：
多克隆
- 保存条件：
低温
- 规格：
0.1 mg
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文献和实验Native purification of His-tagged Proteins from Sf9 cells
Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40 1 mM PMSF 1 µg/ml leupeptin 3. Transfer to oakridge tubes and sonicate
6xHis-tagged protein purification using Qiagen Ni-NTA Column under Native Condit
)Centrifuge lysate at 10,000xg for 20’ at40℃to pellet the cellular debris and save supernatant.Add 5μl 2xSB to 5μl Supernatant and store at 200℃for SDS-PAGE analysisBatch purification under Native ConditionsAdd 2.5 ml of the 50% Ni-NTA slurry to 10 ml cleared
Native purification of His-tagged Proteins from Sf9 cells
Native purification of His-tagged Proteins from Sf9 cells Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0
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