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- 保存条件：
-20℃
- 规格：
50T
产品名称: 全蛋白提取试剂盒 （试用装10T）
产品概述:
全蛋白提取试剂盒（Whole Cell Lysis Assay）可用于从哺乳动物组织和细胞中提取全蛋白，用含有蛋白酶抑制剂的裂解缓冲液Lysis Buffer匀浆裂解组织或细胞，提取过程简便高效。获得的全蛋白可用于Western Blot、IP等后续研究，但不能用于蛋白激酶及磷酸酶免疫共沉淀的研究。
包装信息：
保存条件:
-20℃保存，本试剂盒自订购之日起一年内有效。
该产品被引用文献（仅展示部分）：
1、Zhang H; Zhang S; Dang X, et al., GPNMB plays an active role in the M1/M2 balance. Tissue Cell 2022, 74, 101683.
PubMed: 34800878
2、Gao F; Guo Z; Gao Y, et al., Maternal oxidized soybean oil exposure in rats during lactation damages offspring kidneys via Nrf2/HO-1 and NF-kappaB signaling pathway. J Sci Food Agric 2021.
PubMed: 34791653
3、Zhu G Y; Jia D D; Yang Y, et al., The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System-Interstitial Cells of Cajal-Smooth Muscle Cells Network. J Inflamm Res 2021, 14, 4615-4628.
PubMed: 34552344
4、Wang Y; Chen X; Li J, et al., Quercetin Antagonizes Esophagus Cancer by Modulating miR-1-3p/TAGLN2 Pathway-Dependent Growth and Metastasis. Nutr Cancer 2021, 1-10.
PubMed: 34498538
5、Zhao Y; He L; Wang T, et al., 2-Hydroxypropyl-beta-cyclodextrin Regulates the Epithelial to Mesenchymal Transition in Breast Cancer Cells by Modulating Cholesterol Homeostasis and Endoplasmic Reticulum Stress. Metabolites 2021, 11 (8).
PubMed: 34436503
6、Zhang Y; Xia R; Lv M, et al., Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Front Immunol 2022, 13, 740513.
PubMed: 35350787
7、Du G; Chang S; Guo Q, et al., Protective effects of Tibetan kefir in mice with ochratoxin A-induced cecal injury. Food Research International 2022, 111551.
PubMed: 35840245
操作流程:
Ⅰ细胞蛋白提取
1.贴壁细胞 ：用预冷的1x PBS洗2-3次， 以洗去培养液。
2. 悬浮细胞：将细胞及培养液移至预冷的离心管中，离心10min（1000rpm）后，弃上清。
3. 将预冷的Lysis Buffer按每1ml加入10μl PMSF的比例混匀备用，Lysis Buffer加入量参考下表。
4. 用细胞刮（沉淀振荡）充分破碎细胞，将其倒入离心管中超声；如果没有超声仪，冰上裂解 30min。
5.12000rpm，4℃离心 , 10min，上清为全蛋白提取物，可进行蛋白定量。
6. 分装保存于-70℃，避免反复冻融。
Ⅱ组织蛋白的提取
1. 取新鲜组织 , 用预冷的1xPBS洗2-3次。
2. 将组织剪成小块 , 放入预冷的研钵中 , 倒入液氮充分研碎。
3. 将预冷的Lysis Buffer按每 1ml 加入10μl PMSF的比例混匀备用。
4. 每100mg组织应加入0.5-1ml Lysis Buffer，充分研磨。如果粘度过大则再需加入一定量Lysis Buffer 再次研磨，之后进行超声。
5.12000rpm，4℃离心 , 10min，上清为全蛋白提取物，可进行蛋白定量。
6. 分装保存于-70℃，避免反复冻融。
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文献和实验该产品被引用文献或证书
1、Zhang H; Zhang S; Dang X, et al., GPNMB plays an active role in the M1/M2 balance. Tissue Cell 2022, 74, 101683.
PubMed: 34800878
2、Gao F; Guo Z; Gao Y, et al., Maternal oxidized soybean oil exposure in rats during lactation damages offspring kidneys via Nrf2/HO-1 and NF-kappaB signaling pathway. J Sci Food Agric 2021.
PubMed: 34791653
3、Zhu G Y; Jia D D; Yang Y, et al., The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System-Interstitial Cells of Cajal-Smooth Muscle Cells Network. J Inflamm Res 2021, 14, 4615-4628.
PubMed: 34552344
4、Wang Y; Chen X; Li J, et al., Quercetin Antagonizes Esophagus Cancer by Modulating miR-1-3p/TAGLN2 Pathway-Dependent Growth and Metastasis. Nutr Cancer 2021, 1-10.
PubMed: 34498538
5、Zhao Y; He L; Wang T, et al., 2-Hydroxypropyl-beta-cyclodextrin Regulates the Epithelial to Mesenchymal Transition in Breast Cancer Cells by Modulating Cholesterol Homeostasis and Endoplasmic Reticulum Stress. Metabolites 2021, 11 (8).
PubMed: 34436503
PubMed: 34800878
2、Gao F; Guo Z; Gao Y, et al., Maternal oxidized soybean oil exposure in rats during lactation damages offspring kidneys via Nrf2/HO-1 and NF-kappaB signaling pathway. J Sci Food Agric 2021.
PubMed: 34791653
3、Zhu G Y; Jia D D; Yang Y, et al., The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System-Interstitial Cells of Cajal-Smooth Muscle Cells Network. J Inflamm Res 2021, 14, 4615-4628.
PubMed: 34552344
4、Wang Y; Chen X; Li J, et al., Quercetin Antagonizes Esophagus Cancer by Modulating miR-1-3p/TAGLN2 Pathway-Dependent Growth and Metastasis. Nutr Cancer 2021, 1-10.
PubMed: 34498538
5、Zhao Y; He L; Wang T, et al., 2-Hydroxypropyl-beta-cyclodextrin Regulates the Epithelial to Mesenchymal Transition in Breast Cancer Cells by Modulating Cholesterol Homeostasis and Endoplasmic Reticulum Stress. Metabolites 2021, 11 (8).
PubMed: 34436503
研选
全蛋白提取试剂盒-50T
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