- ¥3196
- promega
- 进口
- V1320
- 2026年05月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
25 reactions
| V1320 | HisLink™ Spin Protein Purification System | 25 reactions | 3,196 |
| V1361 | PDE-Glo™ Phosphodiesterase | 1,000 assays | 5,116 |
| V1362 | PDE-Glo™ Phosphodiesterase | 10,000 assays | 39,795 |
| V1401 | MAO-Glo™ Assay | 200 assays | 2,007 |
| V1402 | MAO-Glo™ Assay | 1,000 assays | 5,240 |
| V1452 | MAO-A | 500µl | 2,581 |
| V1501 | cAMP-Glo™ Assay | 300 assays | 2,842 |
| V1502 | cAMP-Glo™ Assay | 3,000 assays | 15,918 |
| V1503 | cAMP-Glo™ Assay | 30,000 assays | 119,327 |
| V1560 | MAO-Glo™ Assay with MAO-A | 1,000 assays | 6,267 |
| V1621 | Asp-N, Sequencing Grade | 2µg | 1,986 |
| V1651 | Glu-C, Sequencing Grade | 50μg (5 × 10μg) | 3,896 |
| V1671 | rLys-C, Mass Spec Grade | 15µg | 2,089 |
| V1681 | cAMP-Glo™ Max Assay | 2 Plates | 3,720 |
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文献和实验HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
Protein Purification HD exon 1, 16, 38, 56, and 82 CAGs fused with GST. Making constructs. Exon I of HD gene was amplified from partial cDNA constructs obtained from normal (16 CAGs) and patient (48 CAGs). The primers
Purification of GST-fusion protein
Purification of GST-fusion protein This protocol uses Pharmacia glutathione sepharose matrix. Other companies also sells GST beads, e.g. Sigma. 1) Spin down cells and resuspend in ice-cold PBS (40 ml per 1 L of culture). Lyse
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