MHC Class II (RT1D) 抗体 (FITC)

yb114387 产品详细信息
MHC Class II (RT1D) 抗体 (FITC)
anti-MHC Class II (RT1D) antibody (FITC)
抗原
MHC Class II (RT1D)
适用
其他选择 大鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (OX-17)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 3个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品细节 MHC Class II (RT1D) 抗体 (FITC)
免疫原 Rat spleen membrane glycoproteins depleted of Ia-A antigens. Immunocyte Donor: BALB/c spleen Fusion Partner: X63 Ag8.653
克隆位点 OX-17
亚型 IgG1
纯化方法 Protein G Chromatography
目标详细情况 MHC Class II (RT1D) 抗体 (FITC)
别名 MHC Class II RT1D
背景 Synonyms: HLA Class II
使用细节 MHC Class II (RT1D) 抗体 (FITC)
应用备注 Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat Cell Separation Medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 05-0. 1 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: FischerCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsIsotypic Control: FITC Mouse IgG1Cell Source Percentage of cells stained above control: Thymus 10. 2%Spleen 49. 0%Lymph Node 27. 9%
限制 仅限研究用
贮存及处理 MHC Class II (RT1D) 抗体 (FITC)
浓度 0.1 mg/mL
缓冲液 PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
yb-10452R C4b-A补体C4b-A蛋白抗体 浓度1mg/ml
yb-10462R Phospho-CD18 (Thr758)磷酸化整合素β2/Integrin β2抗体 浓度1mg/ml
yb-10461R Phospho-CD18 (Ser756)磷酸化整合素β2/Integrin β2抗体 浓度1mg/ml
yb-15189R C4orf284号染色体开放阅读框28抗体 浓度1mg/ml
yb-10473R CATSPER2阳离子通道精子相关蛋白2抗体 浓度1mg/ml
yb-7136R CATSPER3阳离子通道精子相关蛋白3抗体 浓度1mg/ml
yb-13922R Chorein/VPS13A液泡蛋白分选蛋白VPS13A抗体 浓度1mg/ml
yb-13923R Chp2钙调磷酸酶B相关蛋白2抗体 浓度1mg/ml
yb-13924R CHRAC1CHRAC1蛋白抗体 浓度1mg/ml
yb-13926R CHD9ATP依赖的解螺旋酶抗体 浓度1mg/ml
yb-13927R CXorf6X染色体开放阅读框6抗体 浓度1mg/ml
yb-13928R CHSS2硫酸软骨素酶2抗体 浓度1mg/ml
yb-13929R CHST10碳水化合物磺基转移酶10抗体 浓度1mg/ml
yb-13930R CHST11碳水化合物磺基转移酶11抗体 浓度1mg/ml
yb-13931R CHST12碳水化合物磺基转移酶12抗体 浓度1mg/ml
yb-13932R CHST13碳水化合物磺基转移酶13抗体 浓度1mg/ml
yb-13933R CHST14碳水化合物磺基转移酶14抗体 浓度1mg/ml
yb-13934R CHST2碳水化合物磺基转移酶2抗体 浓度1mg/ml
yb-13935R CHST3碳水化合物磺基转移酶3抗体 浓度1mg/ml
yb-13936R CHST5碳水化合物磺基转移酶5抗体 浓度1mg/ml
yb-13937R CHST6碳水化合物磺基转移酶6抗体 浓度1mg/ml
yb-13916R CHMP7染色质修饰蛋白CHMP7抗体 浓度1mg/ml
yb-13918R CHODL软骨凝集蛋白CHODL抗体 浓度1mg/ml
yb-13919R CCK39胆囊收缩素39抗体 浓度1mg/ml
yb-13921R Choline kinase alpha乙醇胺激酶α抗体 浓度1mg/ml
yb-6422R CATSPER4阳离子通道精子相关蛋白4抗体 浓度1mg/ml
yb-10470R DYNC1I1胞浆动力蛋白中间链1抗体 浓度1mg/ml
yb-10471R DYNC1I2胞浆动力蛋白中间链2抗体 浓度1mg/ml
yb-2686R DKK3抑癌蛋白DKK3抗体 浓度1mg/ml
ybm-0356M Digoxin(2E2)单克隆抗体 浓度1mg/ml
yb-2083R DcR1肿瘤坏死因子配体超家族成员10C 浓度1mg/ml
yb-1863R DNAJC3干扰素诱导蛋白p58ipk抗体 浓度1mg/ml
yb-3659R DACH2转录调节因子DACH2抗体 浓度1mg/ml
yb-2423R DLK1穿膜蛋白DLK1抗体 浓度1mg/ml
yb-2429R DysferlinDysferlin蛋白抗体 浓度1mg/ml
yb-1295R Defensin Beta 2防御素β2/Defensin β2抗体 浓度1mg/ml
yb-1350R DAP5死亡相关蛋白5抗体 浓度1mg/ml
yb-3764R DDX19B解旋酶DEAD5抗体 浓度1mg/ml
yb-2557R DC-SIGN细胞间粘附分子非整合素蛋白3抗体 浓度1mg/ml
yb-0043R DFFBCaspase 激活的脱氧核糖核酸酶抗体 浓度1mg/ml