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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-Vesicle docking protein p115 antibody - N-terminal
- 抗体英文名:
Anti-Vesicle docking protein p115 antibody - N-terminal
- 克隆性:
多克隆
- 规格:
50µg
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
, were prepared. Peptides (containing a Cys residue attached to the N-terminal amino acid) were conjugated to keyhole limpet hemocyanin (KLH) and injected to respective rabbits in the presence of Freund’s complete adjuvant. Following five booster injections
Secretory Vesicle Swelling by Atomic Force Microscopy
with the known water channel inhibitor Hg2+ is accompanied by a reversible loss in both the basal and GTP-stimulable water entry and vesicle swelling. Introduction of AQP1-specific antibody raised against the carboxy-terminal domain of AQP1 blocked GTP-stimulable
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