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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Nitrocellulose Transfer Membrane- 0.22 um
- 抗体英文名：
Nitrocellulose Transfer Membrane- 0.22 um
- 克隆性：
多克隆
- 规格：
10Membrane
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文献和实验Membrane Transfer and Crosslinking for RNA
transfer of nucleic acids onto a membrane. Polyacrylamide gels characteristically have much sharper resolution, but lower loading capacities. The nature of polyacrylamide gel matrices does not permit efficient transfer by passive diffusion
SEMI-DRY ELECTROPHORETlC TRANSFER (WESTERN BLOTS)
that it is just as effective to use a slightly diluted version of the Tris/glycine running buffer used for SDS-polyacrylamide gels. Materials (for one transfer) Power supply capable of 12 - 100 V, 5 W 1 - 7 x 10 cm nitrocellulose sheet, 0.45 or 0.22 um pore
with a maximum of 4 50ml conical tubes. While cell samples are spinning, put 20mls of the 4% BSA PBS into new 50ml conical tubes. When samples are finished spinning, resuspend the pellet in 25mls of PBS. Transfer the sample to the tubes
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