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- 文献和实验
- 技术资料
- 抗体名:
PIWIL3 293T Transfected Lysate
- 抗体英文名:
PIWIL3 293T Transfected Lysate
- 克隆性:
多克隆
- 规格:
100µl
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文献和实验galactosidase assay for cells transfected with pSV- -Galactosidase Vector
Chen-Ming Fan Lab,Carnegie Institute of Washington Prepare cell lysate as you would for Luciferase assay. Take 50uL of lysate and dilute with 100uL of 1X Reporter Lysis buffer (brown bottle at room temp cat#E397A) giving a total volume of 150
Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays
-well microtitre plate to form an array. These vectors are then packaged in lipid-based transfection reagent, cells grown over the top of the array are transfected and the arrays can then be examined for alterations in cellular function as manifested in localised
. As negative control the reaction was also carried out using a non transfected CHO lysate sample. The rate of phenol formation was followed over 2 minute period/270nm/25°C. The activity is expressed in U/L Immuno-reactivity of paraoxonase-1
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