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- promega
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- U1410
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
200µmol(2 × 100µmol each)
| U1410 | Set of dATP, dCTP, dGTP, dTTP | 200µmol(2 × 100µmol each) | 9,759 | |
| U1420 | Set of dATP, dCTP, dGTP, dTTP | 25µmol each | 1,720 | |
| U1511 | dNTP Mix | 200µl | 178 | |
| U1515 | dNTP Mix | 1,000µl | 461 | |
| U2010 | DNA 5' End-Labeling System | 10 reactions | 2,123 | |
| V1061 | Chymotrypsin, Sequencing Grade | 25µg | 1,039 | |
| V1062 | Chymotrypsin, Sequencing Grade | 4 x 25µg | 3,115 | |
| V1071 | Endoproteinase Lys-C, Sequencing Grade | 5µg | 2,012 | |
| V1121 | MEK Inhibitor U0126 | 5mg (5 × 1mg) | 1,769 | |
| V1141 | Anti-ERK 1/2 pAb, Rabbit | 40µl | 2,087 | |
| V1151 | Donkey Anti-Goat IgG, AP | 60µl | 958 | |
| V1161 | SB 203580 | 1mg | 1,016 | |
| V1171 | PMA | 5mg | 1,116 | |
| V1181 | 4α-PMA | 1mg | 743 | |
| V1201 | LY 294002 | 5mg | 1,116 | |
| V1211 | Anti-ACTIVE® p38 pAb, Rabbit, (pTGpY) | 100µl | 3,473 | |
| V1231 | Microtubes, 1.5ml | 1,000/bag | 797 | |
| V1240 | ProFluor® PKA Assay | 4 plate | 6,893 |
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文献和实验: 10 μl 100 mM dATP 10 μl 100 mM dCTP 10 μl 100 mM dGTP 10 μl 100 mM dTTP 3.6 ml ddH2 O Aliquot into 0.5 eppendorf tubes with 10 μl in each tube. To order these nucleotides, call Pharmacia at 1 800-526-3593. Order the dNTP set: 27-2035-01
Make sure that there is enough dCTP and TTP (or dGTP and dATP) in the hot room Set up your PCR master mix. Remember that you need to do a reaction for dCTP and TTP for each sample 10xPCR Buffer 2.5uL
Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
´enzyme buffer (670 mM Tris buffer, 160 mM ammonium sulfate, 40 mM MgCl 2 and 0.1% TweenÒ20), 0.25 mM each of dATP, dGTP, dCTP and dTTP, 1 mM each of primers HBs1 and HBsR1 and 2.0 U of Taq Figure 1. Comparison
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