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- 详细信息
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Carboxypeptidase E (CPE)
- 库存：
100
- 供应商：
上海沪震
羧肽酶E(CPE)重组蛋白 Recombinant Carboxypeptidase E (CPE)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPF322Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 19.7kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
羧肽酶E(CPE)重组蛋白
Subcellular Location n/a
Residues Ala318~Phe476 (Accession # P16870) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- AWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP EETLKTYWED NKNSLISYLE QIHRGVKGFV RDLQGNPIAN ATISVEGIDH DVTSAKDGDY WRLLIPGNYK LTASAPGYLA ITKKVAVPYS PAAGVDFELE SFSERKEEEK EELMEWWKMM SETLNF
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
羧肽酶E(CPE)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPF322Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 19.7kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
羧肽酶E(CPE)重组蛋白
Subcellular Location n/a
Residues Ala318~Phe476 (Accession # P16870) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- AWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP EETLKTYWED NKNSLISYLE QIHRGVKGFV RDLQGNPIAN ATISVEGIDH DVTSAKDGDY WRLLIPGNYK LTASAPGYLA ITKKVAVPYS PAAGVDFELE SFSERKEEEK EELMEWWKMM SETLNF
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
羧肽酶E(CPE)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
：直接进样 HPLC 检测 雅心生物多年来致力于重组蛋白酶的研究，可提供生物医药用酶及相应的申报用资料。测序级肽图用酶如重组胰蛋白酶、重组羧肽酶 B、V8 和重组糜蛋白酶等。
:1。 孵育温度及时间：37℃ 反应 30 分钟。 检测：直接进样 HPLC 检测。 雅心生物多年来致力于重组蛋白酶的研究，可提供生物医药用酶及相应的申报用资料。测序级肽图用酶如重组胰蛋白酶、重组羧肽酶 B、V8 和重组糜蛋白酶等。
Press 3、 Medicinal Protein Engineering Published: December 01, 2008 by CRC Press - 584 Pages Editor(s): Yury E. Khudyakov, Division of Viral Hepatitis, Nat'l Ctr. Infect. Dis., CDCP 4、 Protein Engineering Pub. Date: November
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羧肽酶E(CPE)重组蛋白
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