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- Bio-Rad Laboratories
- MSB1001
- 2025年07月16日
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
is less than 1 mm in diameter. A. Deparaffinization: Xylene 2X 3 mins each. 100% ethanol 2 mins. 95% ethanol 2 mins. 70% ethanol 2 mins. 50% ethanol 2 mins. dH2O 2 mins. B. Staining: 0.1-1% Methyl green
E-Z 96 Fungal DNA Protocol (Centrifugation Processing)
. Seal the E-Z 96® DNA plate with new adhesive film and incubate for 5 min at room temperature. Centrifuge at 4,000 x g for 5 min. 100 ul Elution Buffer is sufficient to elute up to 85% of the DNA from each well of the E-Z 96® DNA plate. A second
1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
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