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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
. 注意事项 1. All steps performed at room temperature. 2. RNA can be stored at –80°C for short time. 3. At step 8, it is needed to prolong the treated time for cell >5 x 106.
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