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- 2025年07月13日
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文献和实验Partial proteolysis with Staph. aureus V8 protease
Materials: Overlay/Rehydration buffer: 125 mM Tris-HCl, pH 6.8 1mM EDTA 0.1% SDS 1mM 2-b-mercaptoethanol 30% glycerol some Bromophenol blue For 1 ml, you will need 0.35 ml of 2X buffer 0.30 ml H20
Genomic (Plant) DNA: Restriction Digestion for Southern Blot
by the 1mm comb and the need to ethanol precipitate the digested DNA in order to end up with a very small volume (~15-20μl) that will fit into the well. If this is the case do the restriction in 25-30μl, skip the ethanol precipitation step and use a 2
µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore
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