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- Bio-Rad Laboratories
- 1704523
- 2025年07月15日
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
per mutagenesis condition 6) Pour cells into petri dish. Expose to UV light source for either 0, 1 or 2 min 7) Collect cells and place in dark 8) Dilute cells 1:100 (10 µl in 990µl water). Further dilute 1:10 for mutagenized cells and 1:20
quantitated in a UV spectrometer. Typically, 1 OD260 (i.e. a solution having an absorbance of one unit at 260 nm with a path length of 1 cm) is said to correspond to a concentration of 30-37 ug per ml. This is for single stranded DNA with an equal
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