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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
5ug
| N1231 | Methylated Human Control | 5ug | 2,739 | |
| N1301 | MethylEdge™ Bisulfite Conversion System | 50 rxn | 3,042 | |
| N1311 | pFN31A Nluc CMV-Hygro Flexi® Vector | 20ug | 2,726 | |
| N1321 | pFN31K Nluc CMV-Neo Flexi® Vector | 20ug | 2,726 | |
| N1331 | pFN32A Nluc CMV-Hygro Flexi® Vector | 20ug | 2,726 | |
| N1341 | pFN32K Nluc CMV-Neo Flexi® Vector | 20ug | 2,726 | |
| N1351 | pNLF1-N [CMV/Hygro] Vector | 20ug | 4,380 | |
| N1361 | pNLF1-C [CMV/Hygro] Vector | 20ug | 4,380 | |
| N1371 | pNLF1-secN [CMV/Hygro] Vector | 20ug | 4,380 | |
| N1381 | pNLF1-HIF1A [CMV/neo] Vector System | 20ug | 6,389 | |
| N1391 | pNLF1-NRF2 [CMV/neo] Vector System | 20ug | 6,389 | |
| N1411 | pNL3.2.CMV[NlucP/CMV] Vector | 20ug | 4,380 | |
| N1441 | pNL1.1.PGK[Nluc/PGK] Vector | 20ug | 4,115 | |
| N1501 | pNL1.1.TK[Nluc/TK]Vector | 20ug | 4,115 |
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文献和实验CpG islands are clusters of CpG dinucleotides that can be found in the 5′ region of about half of human genes (1 ). Methylation of cytosine within the 5′ CpG islands is associated with transcriptional inactivation of the involved gene
Methylated DNA Immunoprecipitation (MeDIP)
that contain methylated cytosines in their genome (human, mouse, Arabidopsis thaliana , Neurospora crassa ). Resuspend the cell pellet or the homogenized tissue in 300 µl TE in a 2 ml eppendorf tube (the volume should be increased for big cell
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
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