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- hz546Ra01
- 2025年07月15日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Adrenergic Receptor Alpha 1A (ADRa1A)
- 库存：
100
- 供应商：
上海沪震
细胞色素P450家族成员11B1(CYP11B1)重组蛋白 Recombinant Cytochrome P450 11B1 (CYP11B1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No. RPD546Ra01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 61.2kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Met1~Val498 (Accession # P15393) with two N-terminal Tags, His-tag and T7-tag
细胞色素P450家族成员11B1(CYP11B1)重组蛋白
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPHR MPLEPWVAHR ELRGLRRGVF LLNGADWRFN RLQLNPNMLS PKAIQSFVPF VDVVARDFVE NLKKRMLENV HGSMSINIQS NMFNYTMEAS HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL PKSLTRWTST RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI VANPQKAMSD LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT FVIIYLYSMG RNPAVFPRPE RYMPQRWLER KRSFQHLAFG FGVRQCLGRR LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV FRFLLMPSSS PFLTFRPV
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
细胞色素P450家族成员11B1(CYP11B1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No. RPD546Ra01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 61.2kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Met1~Val498 (Accession # P15393) with two N-terminal Tags, His-tag and T7-tag
细胞色素P450家族成员11B1(CYP11B1)重组蛋白
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MALRVTADVW LARPWQCLHR TRALGTTAKV APKTLKPFEA IPQYSRNKWL KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPHR MPLEPWVAHR ELRGLRRGVF LLNGADWRFN RLQLNPNMLS PKAIQSFVPF VDVVARDFVE NLKKRMLENV HGSMSINIQS NMFNYTMEAS HFVISGERLG LTGHDLKPES VTFTHALHSM FKSTTQLMFL PKSLTRWTST RVWKEHFDSW DIISEYVTKC IKNVYRELAE GRQQSWSVIS EMVAQSTLSM DAIHANSMEL IAGSVDTTAI SLVMTLFELA RNPDVQQALR QESLAAEASI VANPQKAMSD LPLLRAALKE TLRLYPVGSF VERIVHSDLV LQNYHVPAGT FVIIYLYSMG RNPAVFPRPE RYMPQRWLER KRSFQHLAFG FGVRQCLGRR LAEVEMLLLL HHMLKTFQVE TLRQEDMQMV FRFLLMPSSS PFLTFRPV
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
细胞色素P450家族成员11B1(CYP11B1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
的产量。 细胞凋亡由一系列基因精确地调控，是多细胞生物发育和维持稳态所必需的生理现象。已知凋亡的最终执行者是Caspase家族，它们均为半胱氨酸蛋白酶，各识别一个4氨基酸序列，并在识别序列C端天冬氨酸残基处将底物切断。Caspase含有可被自身识别的序列，可以切割活化自身而导致信号放大，并作用于下游Caspase成员，从而形成Caspase家族的级联放大，最终作用于效应蛋白，引起细胞凋亡。 所以在大规模培养时干扰细胞在培养中凋亡的发生，提高细胞特异性抵制遇到压力而引起凋亡的能力，有利于提高
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细胞色素P450家族成员11B1(CYP11B1)重组蛋白
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