- 询价
- 沪震生物
- 进口/国产
- hz186Hu01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Sucrase Isomaltase (SI)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPD186Hu01
Source Prokaryotic expression
Host E.coli
Purity > 97%
UOM 50ug
Predicted Molecular Mass 18.3kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
蔗糖酶异麦芽糖酶(SI)重组蛋白
Subcellular Location n/a
Residues Asn1717~Ser1827 (Accession # P14410) with two N-terminal Tags, His-tag and S-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- -NQMA QGSLFWDDGE SIDTYERDLY LSVQFNLNQT TLTSTILKRG YINKSETRLG SLHVWGKGTT PVNAVTLTYN GNKNSLPFNE DTTNMILRID LTTHNVTLEE PIEINWS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
蔗糖酶异麦芽糖酶(SI)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验的克隆方法将目的基因克隆进入门载体。 （2）混合包含目的基因的入门克隆和合适的目的载体以及Gateway LR Clonase酶，构建表达克隆。（表达克隆用来在合适的宿主中进行蛋白的表达和分析。） 有几种方法可以构建Gateway入门克隆。无论您选择何种方法，创建的入门克隆都是准备用来与各种目的载体进行重组。 （1）PCR克隆（定向TOPO克隆至入门载体或与供载体B×P重组） （2）限制性内切酶消化和连接进入入门载体 （3）使用pCMV•SPORT6或pEXP-AD502*构建
子干扰RNA片段(small interfering RNAs, siRNAs)。证据表明；一个称为Dicer的酶，是RNase III家族中特异识别双链RNA的一员，它能以一种ATP依赖的方式逐步切割由外源导入或者由转基因，病毒感染等各种方式引入的双链RNA，切割将RNA降解为19-21bp的双链RNAs(siRNAs)，每个片段的3’端都有2个碱基突出。在RNAi效应阶段，siRNA双链结合一个核酶复合物从而形成所谓RNA诱导沉默复合物（RNA-induced silencing complex
【整理】蛋白版2006年06月无人应助帖，请广大战友积极应助，加分从优！
=1&tpg=18&age=0 【求助】请做过P73 wb的战友推荐抗体 http://www.dxy.cn/bbs/post/view?bid=65&id=6346140&sty=1&tpg=18&age=0 【求助】急寻乳糖酶-根皮苷-水解酶（LPH）和蔗糖酶-麦芽糖酶（SI）的抗小鼠抗体 http://www.dxy.cn/bbs/post/view?bid=65&id=6345135&sty=1&tpg=18&age=0 【求助】请问酪蛋白水解物的成分是什么？ http://www
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