Glial Fibrillary Acidic Protei

Glial Fibrillary Acidic Protein (GFAP) (intact) 抗体抗原
Glial Fibrillary Acidic Protein (GFAP)
别名 GFAP, AI836096, cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485
抗原表位
其他选择 intact
适用
其他选择 人, 猪
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (GF-02)
标记
其他选择 This GFAP antibody is un-conjugated
应用范围
其他选择 免疫细胞化学（ICC）, 免疫组织化学石蜡技术（IHC-P）, Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Pubmed 找到1个引用
规格 0.1 mg
发货至 中国 (更改)
发货时间 23至29个工作日
产品编号 yb94316
GFAP抗体: 产品细节 Glial Fibrillary Acidic Protein (GFAP) (intact) 抗体
免疫原 Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
克隆位点 GF-02
亚型 IgM
特异性 The antibody GF-02 exclusively reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system, it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
纯化方法 Purified from ascites by thiophilic adsorption-affinity chromatography and precipitation methods.
纯度 > 95 % (by SDS-PAGE)
GFAP:目标详细情况 Glial Fibrillary Acidic Protein (GFAP) (intact) 抗体
别名 GFAP
背景 Glial Fibrillary Acidic Protein (GFAP) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’,s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
研究领域 Stem Cells, Cytoskeleton, Glia marker, Cell/Tissue Markers, Neurology
使用细节 Glial Fibrillary Acidic Protein (GFAP) (intact) 抗体
应用备注 Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
限制 仅限研究用
贮存及处理 Glial Fibrillary Acidic Protein (GFAP) (intact) 抗体
浓度 1 mg/mL
缓冲液 Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8 °C. Do not use after expiration date stamped on vial label.