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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
40ml
| G9381 | Mammalian Lysis Buffer | 40ml | 530 | |
| G9410 | HaloCHIP™ System | 20 reactions | 5,446 | |
| G9441 | Digitonin | 40µl | 1,125 | |
| G9451 | Protease-Glo™ Assay | 1 each | 11,243 | |
| G9461 | pGloSensor™-10F Linear Vector | 1µg | 5,163 | |
| G9560 | HDAC-Glo™ Class IIa Assay | 10ml | 4,873 | |
| G9590 | HDAC-Glo™ 2 Assay | 10ml | 4,873 | |
| G9651 | pFC14A (HaloTag® 7) CMV Flexi® Vector | 20µg | 2,793 | |
| G9661 | pFC14K (HaloTag® 8) CMV Flexi® Vector | 20µg | 2,793 |
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文献和实验ULTIMATE FREEZE-THAW LYSIS FOR MAMMALIAN CELLS
-abundance proteins (like Myc!).The protocol is described for harvesting mammalian cells from 6 or 10 cm tissue-culture dishes.A.CELL HARVEST.1.Remove media from cells.Wash in ice-cold PBS.2.Scrape cells in 1 ml of ice-cold PBS.Transfer to 1.5 ml microfuge
Electroporation of Antibodies into Mammalian Cells
The introduction into mammalian cells of antibodies with specificities for endogenous cellular factors permits the functional assessment of such factors in the context of living cells. Antibodies have been successfully introduced
Base Excision Repair in Mammalian Cells
A rapid, convenient and safe in vitro assay system for base excision repair is described. Whole cell extracts are prepared by detergent-based cell lysis and provide a vigorous activity of AP site repair. A circular DNA substrate is used
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