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- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
MHC Class II (I-A K,b,d,q,r) 抗体 (FITC)
- 规格：
0.1ml/0.2ml
MHC Class II (I-A K,b,d,q,r)
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (ER-TR3)
标记
其他选择 FITC
应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）
Pubmed 2个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb111989
产品细节 MHC Class II (I-A K,b,d,q,r) 抗体 (FITC)
免疫原 Murine thymic reticulum. Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.
克隆位点 ER-TR3
亚型 IgG2b
无交叉反应 人
交叉反应 （详细） Species reactivity (tested):Mouse (Cells expressing MHC class II antigens.
纯化方法 Affinity Chromatography.
目标详细情况 MHC Class II (I-A K,b,d,q,r) 抗体 (FITC)
背景 MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
研究领域 Major Histocompatibility Complex (MHC), Immunology
使用细节 MHC Class II (I-A K,b,d,q,r) 抗体 (FITC)
应用备注 Flow Cytometry: 10 μg/mL (Use 10 μL of 1/10 diluted antibody to label 10^6 cells). Suggested Positive Control: mouse spleen. Has beed described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 MHC Class II (I-A K,b,d,q,r) 抗体 (FITC)
浓度 0.1 mg/mL
缓冲液 Phosphate buffered saline pH 7.2 (PBS) containing 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store the antibody undiluted at 2-8 °C.
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文献和实验Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
CD4+ T-helper cells recognize antigenic peptides presented by MHC class II molecules. The binding of the nominal peptide to the MHC class II allele is dependent on the amino acid sequence of the peptide as well as on amino acid (aa) residues
Class II MHC Antigen Processing in Phagosomes
(MHC II) molecules and class I MHC (MHC I) molecules to stimulate CD4+ and CD8+ T cells, respectively. To examine the role of phagosomes in processing of antigens for presentation by MHC II molecules to CD4+ T cells, phagosomes have been biochemically
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
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