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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
Ligase I, DNA, ATP-Dependent (LIG1) 抗体
- 规格:
0.1ml/0.2ml
Ligase I, DNA, ATP-Dependent (LIG1)
别名 ATLIG1, DNA ligase 1, T6D22.23, ligI, AL033288, LigI, lig1, wu:fc54a11
适用
其他选择 人
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (10H5)
标记
其他选择 非结合性
应用范围
其他选择 免疫沉淀(IP), Western Blotting (WB)
Pubmed 4个引用可参考
规格 0.1 mg
发货至 中国 (更改)
产品编号 yb136352
产品细节 Ligase I, DNA, ATP-Dependent (LIG1) 抗体
免疫原 Full-length recombinant human DNA Ligase I protein
克隆位点 10H5
亚型 IgG1
片段 Light chain,Chain kappa
交叉反应 小鼠
纯化方法 Protein G affinity purified.. From mouse ascitic fluid..
目标详细情况Ligase I, DNA, ATP-Dependent (LIG1) 抗体
别名 DNA ligase 1
背景 There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.
Synonyms: 126391, MGC130025, P18858, 3978, MGC117397, LIG1, DNA ligase I
分子量 125 kDa
研究领域 DNA/RNA
使用细节 Ligase I, DNA, ATP-Dependent (LIG1) 抗体
应用备注 Western (1:500)
说明
Positive Controls: MCF7
限制 仅限研究用
贮存及处理 Ligase I, DNA, ATP-Dependent (LIG1) 抗体
状态 Liquid
浓度 1 mg/mL
缓冲液 Phosphate-buffered saline, pH 7.2, containing no preservatives
储存液 Without preservative
注意事项 Avoid freeze-thaw cycles.
储存条件 4 °C
储存方法 Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
使RNA的 3′- OH末端与 5′ - 磷酸末端以磷酸二酯键而连结的酶。 EC 6. 5. 1. 3.是 J. Hurwitz等( 1972)从感染 T4 噬菌体的大肠杆菌中提取的,与促进该噬菌体尾部纤维结合的基因 63的蛋白质为同一物质。作为其反应机理,首先是酶与 ATP形成酶 - AMP复合体,复合体的 AMP与 RNA的 5′ - 磷酸末端形成焦磷酸键,它与 RNA的 3′- OH末端形成磷酸二酯键将 AMP游离出。一个 RNA链的 5′与 3′末端结合则生成一个环状分子。对基质
DNA Ligation and Escherichia coli Transformation
DNA ligase, which requires Mg2+ and NAD+ as cofactors and is less commonly used, and T4 DNA ligase produced by the E. coli phage T4, which requires Mg2+ and ATP as cofactors. The in vivo function of both these enzymes is to repair nicks in double
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