Secreted phosphoprotein 1 (SPP

Secreted phosphoprotein 1 (SPP1) 抗体抗原
Secreted phosphoprotein 1 (SPP1)
别名 zgc:111821, BNSP, BSPI, ETA-1, OPN, 2AR, Apl-1, Bsp, Eta, OP, Opn, Opnl, Ric, Spp-1, OSP, AI463453, SPP, SPP1, Spph1, SPPase1
适用
其他选择 犬, 人, 小鼠, 猪, 大鼠
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 酵素免疫分析法（EIA）, Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), 免疫组织化学石蜡技术（IHC-P）
Pubmed 12个引用可参考
规格 0.2 mL
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb118005
产品细节 Secreted phosphoprotein 1 (SPP1) 抗体
免疫原 Synthetic peptide corresponding to Human Osteopontin conjugated to KLH using maleimide.
序列 C-K-S-K-K-F-R-R-P-D-I-Q-Y-P-D
特异性 This antiserum is directed against Human osteopontin. The antibody recognizes the full length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment.
纯化方法 Serum
目标详细情况 Secreted phosphoprotein 1 (SPP1) 抗体
别名 Osteopontin / SPP1
背景 Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during injury/disease processes in patterns overlapping that of OPN. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events.Synonyms: BNSP, Bone Sialoprotein 1, Nephropontin, OPN, SPP-1, Secreted Phosphoprotein 1, Urinary Stone Protein, Uropontin
基因ID 6696
NCBI登录号 NP_000573
UniProt P10451
研究领域 Hormones, Enzymes, Metabolism, Extracellular Matrix
使用细节 Secreted phosphoprotein 1 (SPP1) 抗体
应用备注 This antibody is suitable for Western blotting, Immunohistochemistry (Formalin FixedParaffin-Embedded Sections) and ELISA. A 1/1000 dilution will detect strongly
限制 仅限研究用
贮存及处理 Secreted phosphoprotein 1 (SPP1) 抗体
状态 Liquid
浓度 75 mg/mL (by Refractometry)
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 and 0.01 % (w/v) Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 4 °C/-20 °C
储存方法 Store undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.