Lactate Dehydrogenase A (LDHA)

Lactate Dehydrogenase A (LDHA) 抗体抗原
Lactate Dehydrogenase A (LDHA)
别名 gsd11, ldh-m, ldh1, ldhm, pig19, ldha1, ldhab, LDHC, LDH-M, GSD11, LDH1, LDHM, Ldh1, Ldhm, l7R2, LDH-A, ldh-h, ldha, ldha-B, ldhbb, trg-5
适用
其他选择 兔
宿主
其他选择 山羊
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 酵素免疫分析法（EIA）, 免疫萤光（IF）, 免疫沉淀（IP）, Western Blotting (WB)
Pubmed 3个引用可参考
规格 2 mL
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb116756
产品细节Lactate Dehydrogenase A (LDHA) 抗体
免疫原 Full length lactate dehydrogenase protein isolated from Rabbit muscle.
序列 Rabbit Lactate Dehydrogen ase A chain (muscle), 332 aa, predicted MW 36.6kDa 1 maalkdqlih nllkeehvpq nkitvvgvga vgmacaisil mkdladelal vdvmedklkg 61 emmdlqhgsl flrtpkivsg kdysvtansk lviitagarq qegesrlnlv qrnvnifkfi 121 ipnvvkysph ckllvvsnpv diltyvawki sgfpknrvig sgcnldsarf rylmgerlgv 181 halschgwil gehgdssvpv wsgmnvagvs lktlhpelgt dadkeqwkqv hkqvvdsaye 241 viklkgyttw aiglsvadla esimknlrrv hpistmlkgl ygikedvfls vpcvlgqngi 301 sdvvkvtlts eeeahlkksa dtlwgiqkel qf
特异性 Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
纯化方法 Prepared from monospecific antiserum by delipidation and defibrination
目标详细情况 Lactate Dehydrogenase A (LDHA) 抗体
别名 LDHA
背景 Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59
基因ID 100009107
NCBI登录号 NP_001075746
UniProt P13491
研究领域 Cancer, Metabolism, Enzymes
使用细节 Lactate Dehydrogenase A (LDHA) 抗体
应用备注 Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000). Expect aband approximately 36 kDa in size corresponding to LDH western blotting in theappropriate cell lysate or extract.
限制 仅限研究用
贮存及处理 Lactate Dehydrogenase A (LDHA) 抗体
状态 Liquid
溶解方式 Restore with 2.0 mL of deionized water (or equivalent)
浓度 82 mg/mL (by Refractometry)
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.