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文献和实验A novel method of growing fungi for DNA extraction
). The resulting products were digested for 2 hrs at 37C using HinfI in the buffer supplied with the enzyme. The DNA yields obtained from mycelium grown on RMA were similar to those obtained from mycelium grown in liquid culture. Washing away excess reverse
A novel method of growing fungi for DNA extraction
amplification of the ITS1-ITS4 region of the ribosomal DNA was performed using the primers and conditions given by White et al. (pp. 282-287 In: PCR Protocols, Innis et al. eds Academic Press). The resulting products were digested for 2 hrs at 37C using HinfI
的影响,所以回收率并非是一成不变的。所以Qiagen以严谨的态度提供多种条件下的详细介绍:使用QIAquick回收之前和回收之后再混合的DNA 片段 (大小已指出) 。对所有尺寸的片段均获得了接近80%的回收率。A 1-5 : 回收之前; P : 回收之后再混合。样品使用 1.5% 琼脂糖凝胶分析(TAE buffer)。B 1-3: 回收之前; P : 回收之后混合。样品于 3.5% 高分辨琼脂糖凝胶上分析( TAE buffer)。M : pTZ-HinfI marker。记得《分子克隆II
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