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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Microtubule Associated Protein Tau (MAPt)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB983Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 30.7kDa
Predicted isoelectric point 5.6
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoskeleton. Cell projection, axon
Residues Lys130~Thr386 (Accession # P10636) with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed 微管关联蛋白τ(MAPτ)重组蛋白 below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K VVQEGFLREPGPPGLSHQLM SGMPGAPLLP EGPREATRQP SGTGPEDTEG GRHAPELLKH QLLGDLHQEG PPLKGAGGKE RPGSKEEVDE DRDVDESSPQ DSPPSKASPA QDGRPPQTAA REATSIPGFP AEGAIPLPVD FLSKVSTEIP ASEPDGPSVG RAKGQDAPLE FTFHVEITPN VQKEQAHSEE HLGRAAFPGA PGEGPEARGP SLGEDTKEAD LPEPSEKQPAAAPRGKPVSR VPQLKARMVS KSKDGT
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
微管关联蛋白τ(MAPτ)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验微管结合蛋白(microtubule-associated proteins, MAPs)
与微管特异地结合在一起, 对微管的功能起辅助作用的蛋白质称为微管结合蛋白, 在微管结构中约占10~15%。 一类主要的MAPs家族叫作装配MAPs(assembly MAPs), 作用是将微管在胞质溶胶中进行交联。这些MAPs的结构中具有两个结构域, 一个是碱性的微管蛋白结合结构域, 另一个是酸性的外伸的结构域。 根据序列特点, 将MAPs分成两个主要的类型:Ⅰ型和Ⅱ型(还有其他类型)。MAP1A和MAP1B含有几个重复的氨基酸序列:Lys-Lys-Glu
-CSF和IFN-γ[11]。 CD44是一种跨膜糖蛋白质分子,是T细胞的激活标记,它具有参于信号传递,促进细胞间粘附和细胞迁移等生物学作用。我们结果发现扩增后的粘附性γδT细胞CD44为94%,悬浮生长的γδT细胞为86.39%。Chao等人[9]报告在淋巴结、peyer氏结和表皮内γδΤ细胞CD44的表达具有异质性,我们发现末梢血中γ9δ2Τ细胞也有部分高表达CD44,有关这一亚群的生物学特征有待进一步研究。γ9δ2Τ细胞杀伤肿瘤细胞的细胞毒作用主要是通过穿孔素/颗粒酶途径和Fas/Fasl途径
三句话读懂一篇 CNS:爱的抱抱助力养成「社交牛逼症」?女性肥胖或因爷爷吸烟太早……
availability in bone marrow。 该工作通过分析造血干细胞在稳态以及骨髓再生过程中的表型与功能,揭示了肠道菌群、巨噬细胞和铁元素可获得性之间的相互关联,以及这个全新通路在造血干细胞命运决定中的重要作用! 图 7:来源 Cell Stem Cell 8. Science:微管相关蛋白 MAP7 调控驱动蛋白-1 的结构和功能机制 微管相关蛋白与细胞内转运交流过程紧密相关。 2022 年 1 月 21 日,美国加州大学伯克利分校的 Ahmet Yildiz 和 Eva Nogales
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