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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Interleukin 33 (IL33)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB980Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 21.5kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Ser112~Thr270 (Accession # O95760) with two N-terminal Tags, His-tag and T7-tag
白介素33(IL33)重组蛋白
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-SITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
白介素33(IL33)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验为啥越老越怕冷?竟是免疫细胞「叛变」了!Cell Metab
人员使用 scRNA-seq 数据检测在衰老小鼠中哪些细胞类型表达 Il33,结果发现其 Il33 基因的表达仅限于一群表达 Msln 的细胞群体中,而不是 Pdgfra+细胞。这些数据表明,在衰老过程中,脂肪组织中 IL-33 的细胞来源发生了转换。qPCR 的检测结果显示,与年轻的小鼠相比,老年小鼠的 VAT 中 Il33 的表达总体增加,但皮下脂肪组织中并没有增加。有趣的是,随着 IL-33 的增加,衰老导致编码 IL-33R 的 Il1rl1 转录变体在老年 VAT 中特异性表达增加,而在血清
白细胞介素 (interleukin , IL) 白介素于1979年开始命名,包括淋巴细胞、单核细胞及其他非单个核细胞产生的细胞因子,在细胞间相互作用、免疫调节、造血以及炎症过程中起重要作用。正式命名的白介素,其cDNA基因的克隆和表达均已成功。目前已报道的白介素从ILl到IL33。 几种细胞因子的结构示意图 A.IL-1 B;B.II.-2;C.IL-4;D.IL-10。
向。 07 靶点:IL33 应用:不明原因复发性流产的临床干预 来源:Trophoblast aging driven by IL33 deficiency elevates recurrent pregnancy loss risk through SNAP29 lactylation-mediated autophagy impairment.Autophagy,2026 Jul 图源:10.1080/15548627.2026.2659946[7] 复旦
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