Interleukin 2 Receptor, alpha

Interleukin 2 Receptor, alpha (IL2RA) 抗体 (FITC)抗原
Interleukin 2 Receptor, alpha (IL2RA)
别名 CD25, HEM45, IDDM10, IL2R, TCGFR, IL2RAC, IL-2RA, Il2r, Ly-43
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (7D4)
标记
其他选择 FITC
应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 2个引用可参考
规格 0.5 mg
发货至 中国 (更改)
发货时间 23至28个工作日
产品编号 yb135171
产品细节 Interleukin 2 Receptor, alpha (IL2RA) 抗体 (FITC)
免疫原 IL-2-dependent BALB/c mouse helper T-cell clone HT-2 1
克隆位点 7D4
亚型 IgM
产品特性 The antibody detects: Low affinity chain IL-2 receptor (IL-2R), Mr 55 kDa
The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7. While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antobody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
目标详细情况 Interleukin 2 Receptor, alpha (IL2RA) 抗体 (FITC)
别名 CD25/IL-2Ra
背景 The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.
使用细节 Interleukin 2 Receptor, alpha (IL2RA) 抗体 (FITC)
应用备注 Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
样本量 1 mL
限制 仅限研究用
贮存及处理Interleukin 2 Receptor, alpha (IL2RA) 抗体 (FITC)
缓冲液 Product is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS / NaN3.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件 4 °C