半乳糖 苷果糖检测试剂盒

UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products

Principle:
(β-galactosidase)
(1) Lactulose + H₂O → D-galactose + D-fructose

(glucose oxidase + catalase + H₂O₂)
(2) D-Glucose + H₂O + O₂ → D-gluconic acid + H₂O₂

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(4) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + NADPH
+ CO₂ + H⁺

Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 120 min
Detection limit: 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition: Novel method