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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Chemokine C-C-Motif Receptor 7 (CCR7)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB647Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass n/a
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
趋化因子C-C-基元受体7(CCR7)重组蛋白Residues n/a
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
趋化因子C-C-基元受体7(CCR7)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠趋化因子受体 7(CCR7)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 CCR7 单抗包被于酶标板上,标准品和样品中的 CCR7 与单抗结合,加入生物素化的抗大鼠 CCR7 ,形成免疫复合物连接在板上
受体的表达特征所决定。其中,淋巴滤泡中的B细胞表达CXCR5,识别BLC;T细胞区中的T细胞表达CCR7,识别SLC。而且,B细胞一旦识别抗原,包括识别FDC表面的抗原,则成为CXCR5和CCR7双阳性细胞,因而得以进入(或重新进人)T细胞区与T细胞发生相互作用。然后,如上所述,再返回B细胞区,并在该处大量增殖和分化,形成一种特殊的结构称为生发中心(GC)。因为生发中心必定出现于淋巴滤泡,为此,初级淋巴滤泡演化为次级淋巴滤泡。 B细胞在淋巴结中的迁移和分化受控于不同的趋化因子
趋化因子介导的生物治疗 趋化因子是一类分子质量为8~11kDa的细胞因子样的分泌蛋白,可分为四类:即C-C,C-X-C,C-X3-C和XC趋化因子。其受体属于七次跨膜结构域G蛋白偶联受体。 将不同类型的趋化因子导人肿瘤细胞,能强化宿主抗肿瘤免疫反应。应用方法包括:将趋化因子基因导人肿瘤细胞,将重组蛋白注射人肿瘤,将趋化因子与肿瘤抗原结合的融合蛋白注入肿瘤,或者将趋化因子基因转导入DC或基质细胞。 使用单一趋化因子的疗法 将CCLl、CCL
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