- ¥34595
- promega
- 进口
- E2312
- 2025年12月09日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
5 X 1ml
| E2312 | FuGENE® HD Transfection Reagent | 5 X 1ml | 34,595 |
| E2431 | TransFast™ Transfection Reagent | 1.2mg | 2,600 |
| E2440 | CheckMate™ Mammalian Two-Hybrid System | 1 system | 5,871 |
| E2510 | Steady-Glo® Luciferase Assay System | 10ml | 854 |
| E2520 | Steady-Glo® Luciferase Assay System | 100ml | 6,372 |
| E2550 | Steady-Glo® Luciferase Assay System | 10 × 100ml | 54,209 |
| E2610 | Bright-Glo™ Luciferase Assay System | 10ml | 1,058 |
| E2620 | Bright-Glo™ Luciferase Assay System | 100ml | 6,941 |
| E2650 | Bright-Glo™ Luciferase Assay System | 10 × 100ml | 61,042 |
| E2661 | Glo Lysis Buffer, 1X | 100ml | 532 |
| E2691 | FuGENE® 6 Transfection Reagent | 1ml | 4,135 |
| E2692 | FuGENE® 6 Transfection Reagent | 5 X 1ml | 16,838 |
| E2693 | FuGENE® 6 Transfection Reagent | 0.5ml | 2,386 |
| E2710 | Renilla-Glo® Luciferase Assay System | 10ml | 2,323 |
| E2720 | Renilla-Glo® Luciferase Assay System | 100ml | 8,446 |
| E2750 | Renilla-Glo® Luciferase Assay System | 10 X 100ml | 77,252 |
| E2810 | Renilla Luciferase Assay System | 100 assays | 1,207 |
| E2820 | Renilla Luciferase Assay System | 1,000 assays | 5,841 |
| E2920 | Dual-Glo® Luciferase Assay System | 10ml | 2,179 |
| E2940 | Dual-Glo® Luciferase Assay System | 100ml | 12,887 |
| E2980 | Dual-Glo® Luciferase Assay System | 10 × 100ml | 114,175 |
| E3030 | Primer Extension System-AMV Reverse Transcriptase | 40 reactions | 1,617 |
| E3050 | Gel Shift Assay Core System | 100 reactions | 2,830 |
| E3091 | HeLaScribe® Nuclear Extract in vitro Transcription Grade | 40 reactions | 3,456 |
| E3092 | HeLaScribe® Nuclear Extract in vitro Transcription Grade | 160 reactions | 9,302 |
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- 询问日期
文献和实验【求助】用fugene 做过转染的进来！！！参与者：yinqiscott小弟准备用罗氏公司的fugene HD来做细胞转染，但是一点不明白，在载体和质粒无血清孵育15分钟后，加到细胞里，这时候的培养液应该用无血清的还是有血清的呢???参与者：shilly我们用的是fugene 6，加到细胞里面的时候是有血清的培养液说明书上说不影响，如果要换无血清培养液也要几个小时以后换。参与者：scmfz明天准备做，老板让我用有血清的，但无抗菌素的。参与者：cachenuse serum-free
Transient Transfection and Adrenergic Receptor Promoter Analysis
well for transfection of β 1 -AR gene fragments into adherent cell lines, but is much less effective for transfection of DNA into primary cultures. As noted by other investigators ( 5 ), we have applied FuGENE™ 6 transfection reagent, a nonliposomal lipid compound
High-Efficiency Nonviral Transfection of Primary Chondrocytes
. In addition to the primary protocol, we present two additional reliable, albeit less efficient backup protocols, the first using exponential decay electroporation and the second FuGENE™ 6 transfection.
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