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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
DNA Helicase B (HELB) (N-Term) 抗体
- 规格:
0.1ml/0.2ml
DNA Helicase B (HELB)
别名 RGD1560103, hDHB, AI447783, D10Ertd664e
抗原表位
其他选择 N-Term
适用
其他选择 人
宿主
其他选择 兔
克隆类型
多克隆
应用范围
其他选择 Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品编号 yb183353
产品细节 DNA Helicase B (HELB) (N-Term) 抗体
免疫原 Synthetic peptide directed towards the N terminal of human HELB
序列 FGRFPITGAW WRVKVQVKPV VGSRSYQYQV QGFPSYFLQS DMSPPNQKHI
预测反应 Human : 100 %, Pig : 80 %
产品特性 This is a rabbit polyclonal antibody against HELB. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Protein A purified
目标详细情况 DNA Helicase B (HELB) (N-Term) 抗体
别名 HELB
背景 The protein encoded by this gene is a DNA helicase. A dominant-negative mutant of this protein blocks chromosomal DNA replication and suggests that its function is required for S phase entry.
分子量 120 kDa
基因ID 92797
NCBI登录号 NP_387467, NM_033647
UniProt Q8NG08
使用细节 DNA Helicase B (HELB) (N-Term) 抗体
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 1087 AA
限制 仅限研究用
贮存及处理 DNA Helicase B (HELB) (N-Term) 抗体
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
图像 引用 贮存及处理 使用细节 目标详细情况 回页首
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anti-DNA Helicase B (HELB) (N-Term) antibody
WB Suggested Anti-HELB Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate
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文献和实验Searching for Non‐B DNA‐Forming Motifs Using nBMST (Non‐B DNA Motif Search Tool)
., and Stephens, R.M. 2011. Non‐B DB: A database of predicted non‐B DNA‐forming motifs in mammalian genomes. Nucleic Acids Res. 39:D383‐D391. Cooper, D.N., Bacolla, A., Ferec, C
Syntheses of DNA Duplexes That Contain a N4C‐Alkyl‐N4C Interstrand Cross‐Link
., and Miller, P.S. 2002b. N(4)C‐alkyl‐N(4)C cross‐linked DNA: Bending deformations in duplexes that contain a ‐CNG‐ interstrand cross‐link. Biochemistry 41:8605‐8612. Rajski, S.R
Mini DNA extraction from wheat leaves for PCR and Southern B
O. After adding water, set tubes upright on shaker overnight to allow your DNA to be resuspended. This long treatment will also result in the remaining RNA being degraded.......(100-200 mg, might yield 200 ug of DNA). 14. Store at 4 ℃. (long term at -20 ℃
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