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- 沪震生物
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- hz599Mu01
- 2025年07月05日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Granzyme A (GZMA)
- 库存：
100
- 供应商：
上海沪震
颗粒酶A(GZMA)重组蛋白 Recombinant Granzyme A (GZMA)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPA599Mu01
Source Prokaryotic expression
Host E.coli
Purity > 97%
UOM 50ug
Predicted Molecular Mass 27.2kDa
颗粒酶A(GZMA)重组蛋白Predicted isoelectric point 9.4
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location Secreted; Cytoplasmic granule
Residues Ile29~Val260 (Accession # P11032) with a N-terminal His-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with a N-terminal His-tag, its sequence is listed below.
MGHHHHHHSGSEF-II GGDTVVPHSR PYMALLKLSS NTICAGALIE KNWVLTAAHC NVGKRSKFIL GAHSINKEPE QQILTVKKAF PYPCYDEYTR EGDLQLVRLK KKATVNRNVA ILHLPKKGDD VKPGTRCRVA GWGRFGNKSA PSETLREVNI TVIDRKICND EKHYNFHPVI GLNMICAGDL RGGKDSCNGD SGSPLLCDGI LRGITSFGGE KCGDRRWPGV YTFLSDKHLN WIKKIMKGSV
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
颗粒酶A(GZMA)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPA599Mu01
Source Prokaryotic expression
Host E.coli
Purity > 97%
UOM 50ug
Predicted Molecular Mass 27.2kDa
颗粒酶A(GZMA)重组蛋白Predicted isoelectric point 9.4
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location Secreted; Cytoplasmic granule
Residues Ile29~Val260 (Accession # P11032) with a N-terminal His-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with a N-terminal His-tag, its sequence is listed below.
MGHHHHHHSGSEF-II GGDTVVPHSR PYMALLKLSS NTICAGALIE KNWVLTAAHC NVGKRSKFIL GAHSINKEPE QQILTVKKAF PYPCYDEYTR EGDLQLVRLK KKATVNRNVA ILHLPKKGDD VKPGTRCRVA GWGRFGNKSA PSETLREVNI TVIDRKICND EKHYNFHPVI GLNMICAGDL RGGKDSCNGD SGSPLLCDGI LRGITSFGGE KCGDRRWPGV YTFLSDKHLN WIKKIMKGSV
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
颗粒酶A(GZMA)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
-2，4，5，6，10，12等）、颗粒酶（Granzyme B）、肿瘤坏死因子（TNF-α）。目前全球ELISPOT试剂盒的主要供应商有：荷兰U-CyTech公司、瑞典Mebtech公司、法国Diaclone公司、美国BD Pharmingen公司和R&D公司。他们的产品各有特点，其中荷兰U-CyTech公司的产品质优价廉，在国内市场居于主导地位。（3) 多细胞因子的检测通常的ELISPOT检测每个孔只检测一种细胞因子。如果要在一个ELISPOT孔内同时检测两种或者两种以上的细胞因子，可以包被两种抗体以捕获两种
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颗粒酶A(GZMA)重组蛋白
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