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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Vitamin D Receptor (VDR)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPA475Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass n/a
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
维生素D受体(VDR)重组蛋白Residues n/a
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
维生素D受体(VDR)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人维生素D 受体 ( VDR )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 VDR 单抗包被于酶标板上,标准品和样品中的 VDR与单抗结合,加入生物素化的抗人 VDR ,形成免疫复合物连接在板上,辣根
1.小硕我正在做关于维生素D3受体(VDR)多态性实验,准备用BsmI酶切 2.文献上多用Morrison的经典引物:P15′CAA CCA AGA CTA CAA GTA CCG CGT CAG TGA3′和P2AAC CAG CGG GAA GAG GTC AAG GG3 3.但是P2在pubmed上BLAST后如下 |74268229|gb|BC103387.1| Bos taurus similar to calcium acti... 34.2 4.1 gi
吴佰霖 老板的一个课题,一个分泌性蛋白,可结合一些小分子,与膜上受体结合,但是用重组表达的蛋白与 pcDNA过表达这个蛋白的生物学效果不一样 请问有什么研究策略吗 freecell 你的研究材料? 研究目的? 吴佰霖 材料:细胞系,酵母表达重组蛋白,pcDNA表达克隆载体, 研究目的是分泌性蛋白结合受体后在胞内的信号通路,或相关作用分子
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