All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: C2C12 whole cell lysateLane 3: Hela whole cell lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: A431 whole cell lysateLane 3: C2C12 whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: NIH/3T3 whole cell lysateLane 3: A431 whole cell lysateLane 4: C2C12 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: C6 whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: A431 whole cell lysateLane 3: C2C12 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: C6 whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RAB7 Antibody (C-term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: C6 whole cell lysateLane 3: Hela whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RAB7 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
RAB7 Antibody (C-term) (Cat. #AP8665b) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.