- 询价
- 沪震生物
- 中国/美国
- hz145Hu01
- 2025年09月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Vascular Endothelial Growth Factor C (VEGFC)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPA145Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 45.5kDa
Predicted isoelectric point 8.2
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
血管内皮生长因子C(VEGFC)重组蛋白Subcellular Location Secreted
Residues Glu47~Trp413 (Accession # P49767) with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EATA YASKDLEEQL RSVSSVDELM TVLYPEYWKM YKCQLRKGGW QHNREQANLN SRTEETIKFA AAHYNTEILK SIDNEWRKTQ CMPREVCIDV GKEFGVATNT FFKPPCVSVY RCGGCCNSEG LQCMNTSTSY LSKTLFEITV PLSQGPKPVT ISFANHTSCR CMSKLDVYRQ VHSIIRRSLP ATLPQCQAAN KTCPTNYMWN NHICRCLAQE DFMFSSDAGD DSTDGFHDIC GPNKELDEET CQCVCRAGLR PASCGPHKEL DRNSCQCVCK NKLFPSQCGA NREFDENTCQ CVCKRTCPRN QPLNPGKCAC ECTESPQKCL LKGKKFHHQT CSCYRRPCTN RQKACEPGFS YSEEVCRCVP SYW
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
血管内皮生长因子C(VEGFC)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验the reconstituted vial to sit at 4oC for at > 2 hours before use. (将重悬液在4oC静置2小时以上) 3. This solution can be stored at 2-8oC for up to 1 week. 第 3 步：该重悬液在 2-8oC 最长可保存 1 周。 用说明书上推荐的溶液将细胞因子或重组蛋白重悬到推荐的浓度后，细胞因子或重组蛋白可放置在2-8o C，即冰箱的冷藏室。在这种条件下，细胞因子或重组蛋白的活性
can be stored at 2-8oC for up to 1 week. 第 3 步：该重悬液在 2-8oC 最长可保存 1 周。 用说明书上推荐的溶液将细胞因子或重组蛋白重悬到推荐的浓度后，细胞因子或重组蛋白可放置在2-8o C，即冰箱的冷藏室。在这种条件下，细胞因子或重组蛋白的活性最长可保持1周。这对一个周期为5-7天的实验，如DC(树突状细胞)的诱导成熟是足够的。在此期间，只要每次从冰箱里吸取一定量的细胞因子或重组蛋白溶液加入到培养体系内即可。 其实，说明书上推荐的浓度
因为短的肽标签对目标蛋白的结构影响最小，而大的亲和标签可能限制重组蛋白的折叠，影响蛋白质的生物学功能。（2）亲和标签对蛋白质稳定性的影响，亲和标签的加入是否会影响到目标蛋白的正确折叠，是促进目标蛋白的可溶性，还是容易形成包涵体，会不会造成氨基酸C和N端破坏，使目的蛋白表达不完全。（3）亲和标签所融合的位置，亲和标签可以加在N端，可以加在C端，也进行串联。多数情况下蛋白质的N端区域对蛋白质的功能不是太重要，所以在N端进行融合标签的融合常常能保留蛋白质的生物学功能。由于N端DNA序列对蛋白质的转录
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