- 询价
- 沪震生物
- 中国/美国
- hz133Hu01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Tumor Necrosis Factor Alpha (TNFa)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPA133Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 21.0kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Val77~Leu233 with two N-terminal Tags, His-tag and T7-tag
肿瘤坏死因子α(TNFα)重组蛋白 Formulation Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
VRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
肿瘤坏死因子α(TNFα)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Immunohistochemical Localization of TNFα and Its Receptors in the Rodent Central Nervous System
The protocols for immunohistochemical localization of tumor necrosis factor α (TNFα), TNFRp55, and TNFRp75 in central nervous system (CNS) slices from laboratory animals are described. The procedure requires intracardiac perfusion of animals
Assessment of TNFα-Induced Endothelial Damage Through the Loss of Its Barrier Function
Tumor necrosis factor α (TNFα) appears to be the main mediator causing the systemic manifestations of sepsis and septic shock associated with edema (1 ,2 ). In vitro it augments movement of permeability tracers across endothelial cell
，或者可以表达以产生重组蛋白。pET-32α（+）载体系列用于表达与109 aa Trx•Tag™硫氧还蛋白融合的肽序列。它属于一种表达系统，可在其激活后产生所需的蛋白质。插入原核宿主细胞。原核细胞如大肠杆菌因为它们廉价，快速的生物量积累和简单的工艺规模扩大，因此是外源蛋白表达的首选宿主。在本文中，我们将重点总结这种在我们的实验室中使用的有用技术。表达后pET-32α（+）载体的构建，蛋白质表达和蛋白质纯化将在本文中详细描述。获得用于构建载体的外源DNA片段插入载体的DNA通常可以使用聚合酶链式反应（PCR
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