- 询价
- 沪震生物
- 中国/美国
- hz099Ca01
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Matrix Metalloproteinase 13 (MMP13)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Canis familiaris; Canine (Dog)
Product No. RPA099Ca01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues n/a
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
基质金属蛋白酶13(MMP13)重组蛋白STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
基质金属蛋白酶13(MMP13)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验M/NM_000632 calmodulin 2/NM_001743 nuclear mitotic apparatus protein 1/NM_006185 oligodendrocyte lineage transcription factor 2/NM_005806 Mmp13 Mmp1 [url=]http://www.dxy.cn/bbs/thread/1315321CEA [/url] GAPDH cyclin-dependent kinase inhibitor
2008 Publisher: Springer-Verlag New York, LLC Series: Nucleic Acids and Molecular Biology Series , #22 ISBN-13: 9783540709374 ISBN: 3540709371
)，最终结果还是部分蛋白未溶解，导致蛋白活性的减弱。 3) 一定不能振荡(vortex)。 这里所说的振荡是指用涡旋仪进行快速振荡。 有用户会问，若想保证溶解液与冻干粉充分混匀，以实现细胞因子或重组蛋白的完全溶解，该怎么办呢？ 答：多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般我们用移液枪的枪头轻吹几下，即可使细胞因子或重组蛋白完全溶解。 对于不易溶解的细胞因子或蛋白，PeproTech会在说明书中进行备注(Note)。如在重组人IL-13(产品
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