- 询价
- 沪震生物
- 中国/美国
- hz045Ca01
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Canis familiaris; Canine (Dog)
Product No. RPA045Ca01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues n/a
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% 粒细胞巨噬细胞集落刺激因子(GMCSF)重组蛋白 trehalose, 0.01% sarcosyl.
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
粒细胞巨噬细胞集落刺激因子(GMCSF)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验年耿信笃教授首先将高效疏水相互作用色谱(HPHIC)用于变性蛋白的复性，很好的解决了上述问题，现已成功用于重组人干扰素-g(rhIFN-g)、重组人干扰素-a(rhIFN-a)、人粒细胞集落刺激因子(rhG-CSF)、重组人胰岛素原(proinsulin)、重组牛朊病毒(prion)等重组蛋白以及溶菌酶和核搪核酸酶等标准模型蛋白的复性与同时纯化中。目前，排阻色谱法、离子交换色谱法和亲合色谱法也已用于蛋白质的复性和同时纯化中。与传统的稀释法及透析法比较，用色谱法进行蛋白复性的优点是：①在进样后可很快
/α亚族，主要趋化中性粒细胞，包括IL-8、黑素瘤细胞生长刺激活性(GRO/MGSA）、血小板因子-4（PF-4）、血小板碱性蛋白、蛋白水解来源的产物CTAP-III和β-thromboglobulin、炎症蛋白10（IP-10）、ENA-78；（2）C-C/β亚族，主要趋化单核细胞，包括巨噬细胞炎症蛋白1α（MIP-1α）、MIP-1β、RANTES、单核细胞趋化蛋白-1（MCP-1/MCAF）、MCP-2、MCP-3和I-309。 7.其它细胞因子，如表皮生长因子（EGF）、血小板衍生
Expression on Eosinophils by p38 Mitogen-Activated Protein Kinase and Nuclear Factor-κB 白细胞介素-3,-5，和粒巨噬细胞集落刺激因子（GMCSF）通过p38丝裂原活化蛋白激酶（MAPK）和核转录因子NFκB诱导嗜酸性粒细胞中黏附分子的表达 嗜酸性粒细胞是一种重要的炎症效应细胞，它通常集聚在变应性炎症部位如气道粘膜，被激活的嗜酸性粒细胞释放细胞毒性分子。嗜酸性粒细胞的细胞膜上表达有多种黏附分子如免疫球蛋白（Ig）超家族
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