- 询价
- 沪震生物
- 中国/美国
- hz025Bo01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Eosinophil Chemotactic Factor (ECF)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Bos taurus; Bovine (Cattle)
Product No. RPA025Bo01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 12.3kDa
Applications SDS-PAGE; WB; ELISA; IP.
嗜酸粒细胞趋化因子(ECF)重组蛋白Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Gln24~Ser97 with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
QPASIPT ICCFNMSKKK ISIQRLQSYR KITSSKCPQK AVIFNTKQNK KICVDPQEKW VQNAMEYLNQ KSQTLKS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation 嗜酸粒细胞趋化因子(ECF)重组蛋白test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验趋化因子介导的生物治疗 趋化因子是一类分子质量为8～11kDa的细胞因子样的分泌蛋白，可分为四类：即C-C，C-X-C，C-X3-C和XC趋化因子。其受体属于七次跨膜结构域G蛋白偶联受体。 将不同类型的趋化因子导人肿瘤细胞，能强化宿主抗肿瘤免疫反应。应用方法包括：将趋化因子基因导人肿瘤细胞，将重组蛋白注射人肿瘤，将趋化因子与肿瘤抗原结合的融合蛋白注入肿瘤，或者将趋化因子基因转导入DC或基质细胞。 使用单一趋化因子的疗法 将CCLl、CCL
上可形成直径为数厘米的区域。随后，疹块边缘的血管扩张、充血形成特征性的红框，称为风团。20mid内皮肤出现典型的风团或发生呼吸道支气管痉挛，是临床上I型速发型超敏反应的典型表现。这种反应可被多种血液中的生物活性物质所扩大和增强，如血小板活化因子(PAF)可促使血小板聚集从而释放组胺、肝素和血管活性胺等。嗜酸粒细胞趋化因子(ECF-A)和中性粒细胞趋化因子可分别使得嗜酸粒细胞和中性粒细胞向局部趋化，释放多种水解敢�鹱橹�邓馈?/DIV> 2．迟发相反应(1atephasereaction
翌圣HiActi®细胞因子助力Cell研究，解析脑膜调控大脑皮层构建的关键机制
the spatiotemporal developmen to fhuman meninges”为题于2026年5月在Cell上在线发表。 01 翌圣助力产品 在该研究中，研究团队使用翌圣生物Recombinant Human CXCL12/SDF-1 alpha Protein 重组人CXCL12/SDF-1α（90914ES）作为Transwell迁移实验中的阳性趋化因子对照，使用浓度为100 ng/mL。 CXCL12作为经典趋化因子，可用于验证M2巨噬细胞对趋化信号
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