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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
Red Fluorescent Protein (RFP) (AA 234) 抗体 (Biotin)
- 规格：
0.1ml/0.2ml
Red Fluorescent Protein (RFP)
别名 RFP, RNF76
抗原表位
其他选择 AA 234
适用
其他选择 Discosoma
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 Biotin
应用范围
其他选择 Western Blotting (WB), 酶联免疫吸附测定（ELISA）, Fluorescence Microscopy (FM)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 23至28个工作日
产品编号 yb129733
产品细节 Red Fluorescent Protein (RFP) (AA 234) 抗体 (Biotin)
免疫原 The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant
亚型 IgG
产品特性 Concentration Definition: by UV absorbance at 280 nm
纯化方法 Pre-adsorbed
目标详细情况 Red Fluorescent Protein (RFP) (AA 234) 抗体 (Biotin)
别名 RFP
背景 Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
研究领域 Tags/Labels
使用细节Red Fluorescent Protein (RFP) (AA 234) 抗体 (Biotin)
应用备注 Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
限制 仅限研究用
贮存及处理 Red Fluorescent Protein (RFP) (AA 234) 抗体 (Biotin)
状态 Lyophilized
溶解方式 Restore with deionized water (or equivalent)
浓度 1.1 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
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文献和实验. We then developed a color-coded imaging model that can visualize the interaction between αv integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam� vascularized after implantation in red fluorescent protein (RFP
A transgenic mouse, in which the regulatory elements of the stem cell marker, nestin drive green fluorescent protein (ND-GFP), expresses GFP in nascent blood vessels. Red fluorescent protein (RFP)-expressing tumors transplanted to nestin-GFP
(Walter Steffen) Protein (Antibody) Fluorescent Labeling (Mario Roederer) Detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols
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