Recombinant Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)
库存:
100
供应商:
上海沪震
肿瘤坏死因子α诱导蛋白6(TNFαIP6)重组蛋白Recombinant Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPM405Mu01
Source Prokaryotic expression
Host E.coli
Purity > 90%
UOM 50ug
Predicted Molecular Mass 32.7KDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Trp18~Leu275 with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
肿瘤坏死因子α诱导蛋白6(TNFαIP6)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
WGF KNGIFHNSIW LEQAAGVYHR EARAGRYKLT YAEAKAVCEF EGGRLATYKQ LEAARKIGFH VCAAGWMAKG RVGYPIVKPG PNCGFGKTGI IDYGIRLNRS ERWDAYCYNP HAKECGGVFT DPKRIFKSPG FPNEYDDNQV CYWHIRLKYG QRIHLSFLDF DLEHDPGCLA DYVEIYDSYD DVHGFVGRYC GDELPEDIIS TGNVMTLKFL SDASVTAGGF QIKYVTVDPA SKSSQAKNTS TTGNKKFLPG RFSHL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
肿瘤坏死因子α诱导蛋白6(TNFαIP6)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.