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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Steroid 5 Alpha Reductase 1 (SRD5a1)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. RPM286Mu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass n/a
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
类固醇5α还原酶1(SRD5α1)重组蛋白Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
Residues n/a
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity 类固醇5α还原酶1(SRD5α1)重组蛋白to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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- 内容
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文献和实验(1)升高:感染性疾病(细菌性、病毒性)、恶性肿瘤、胶原病、妊娠、外科手术、药物(雌激素、口服避孕药、肾上腺类固醇、前列腺素等),斑疹伤寒等。 医学教育|网搜集整理 (2)降低:α1-AT缺乏症、新生儿呼吸窘迫综合征、重症肝炎、肾病综合征、蛋白丧失性胃肠症、营养不良、未成熟儿、肾移植早期排斥反应等。
内源性FSTL1 通过激活钠‐钾泵引起膜超极化调节痛觉信息传递
分泌到胞外,NKA是一种跨膜蛋白。因此推断,FSTL1 可能是与NKA的胞外部分结合。研究者合成了NKA α1 亚基在胞外的肽段,用BIAcore 分析这些肽段与FSTL1 的结合能力,发现M3M4 和M7M8 肽段与FSTL1 的结合能力较强。进一步的细胞实验发现M3M4 和M7M8 部位有突变的α1 亚基与FSTL1 结合能力较弱,从另一个侧面证明了以下结论:FSTL1 正是通过与M3M4 和M7M8 胞外肽段与NKA α1 亚基结合。 而在培养的DRG 神经元中加入FSTL1 重组蛋白
2+ 依赖方式刺激ACTH及β-END的释放,可强CRH促进ACTH分泌的活性,增加GH及PRL的分泌。thymosin V还可调节大鼠卵巢颗粒合成类固醇激素及分泌IL-6。 (2)thymolymphotropin:刺激大鼠PRL及皮质酮的分泌。 (3)thymulin:对大鼠皮质酮的基础分沁无刺激作用,同样thymosin α1,thymosin β4也不影响ACTH的释放。MB35在离体条件下可有效地刺激GH及PRL分泌。
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