- 询价
- 沪震生物
- 进口/国产
- hz307Hu01
- 2025年07月13日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPL307Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 59.6kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Ile30~Lys511 with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
I DETRAHLLLK EKMMRLGGRL VLNTKEELAN ERLMTLKIAE MKEAMRTLIF PPSMHFFQAK HLIERSQVFN 猫眼综合征染色体区候选基因1(CECR1)重组蛋白ILRMMPKGAA LHLHDIGIVT MDWLVRNVTY RPHCHICFTP RGIMQFRFAH PTPRPSEKCS KWILLEDYRK RVQNVTEFDD SLLRNFTLVT QHPEVIYTNQ NVVWSKFETI FFTISGLIHY APVFRDYVFR SMQEFYEDNV LYMEIRARLL PVYELSGEHH DEEWSVKTYQ EVAQKFVETH PEFIGIKIIY SDHRSKDVAV IAESIRMAMG LRIKFPTVVA GFDLVGHEDT GHSLHDYKEA LMIPAKDGVK LPYFFHAGET DWQGTSIDRN ILDALMLNTT RIGHGFALSK HPAVRTYSWK KDIPIEVCPI SNQVLKLVSD LRNHPVATLM ATGHPMVISS DDPAMFGAKG LSYDFYEVFM GIGGMKADLR TLKQLAMNSI KYSTLLESEK NTFMEIWKKR WDKFIADVAT K
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
猫眼综合征染色体区候选基因1(CECR1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验师们的魔法棒，在现代技术中则变身为科学家们的实力宠儿。它是酵母菌中最早用于基因克隆和表达的宿主，于1996年其全基因组测序工作完成，遗传背景清晰。该表达系统主要有两种类型的载体，包括整合型载体和附加型载体。 整合型载体不含酵母DNA复制起始区，不能在酵母细胞内自主复制。表达载体转到酵母细胞后借助其上的整合区元件与宿主染色体发生同源重组，随染色体复制而进行复制和表达。稳定性高，只是拷贝数低下。像AtaGenix拥有的pATX3载体则将整合区设定在酿酒酵母核糖体DNA（rDNA）基因簇上，转化重组
蛋白。诱导的浓度的高低对目的蛋白的表达也会有产生影响。BL21（DE3）, Rosetta(DE3)等大肠杆菌表达系统这些菌株都敲除了蛋白酶，并溶源了噬菌体DE3。DE3是的一种衍生λ噬菌体，带有噬菌体21抗性区和 lacI基因，lacUV5启动子，以及 T7 RNA聚合酶基因。这一区段被插入int基因，因此阻止了DE3在没有辅助噬菌体时整合到染色体上或从染色体切出。一旦形成DE3溶原状态，就只有受IPTG诱导的lacUV5启动子指导T7 RNA聚合酶基因转录，在溶原培养体系中加入IPTG诱导T
3敢和PD-1并驾齐驱，TIM3到底是何方神圣? TIM全名T淋巴细胞免疫球蛋白黏蛋白（T cell immunoglobulin mucin），还有个洋气的名字CD366，是2002年money等[6]在研究哮喘易感基因的过程中发现的。人类TIM家族只包括TIM1、TIM3和TIM4，位于5号染色体5q33.3上，TIM分子的共同结构包括：免疫球蛋白N末端可变区、粘蛋白结构域、跨膜区和胞内区。 图片来源于：http://www.genecards.org/cgi
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