碱性磷酸酶(Sigma代理)P0114-10KU

磷酸酶(碱性) 来源于牛肠粘膜

Phosphatase, Alkaline from bovine intestinal mucosa

BioUltra, buffered aqueous glycerol solution, ≥6,500 DEA units/mg protein

产品名称
Analysis Note
包装规格取决于 DEA 单位

General description
Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. 3

Unit Definition
在 pH 9.8 和 37°C 下，一 DEA 单位本品每分钟可水解 1μmol 4-硝基苯基磷酸酯。（一甘氨酸单位相当于 ~3 DEA 单位）

Physical form
Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0

Application
High specific activity grade recommended for antibody and protein conjugation.

Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation.

碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性​磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化，以防止自我连接。经碱性磷酸酶​的去磷酸化作用后，DNA 或 RNA 还可被放射性标记磷酸盐标记（通过 T4 多核苷酸激酶）。

Biochem/physiol Actions
Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A. 1,2

该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛​的特异性。常用于蛋白质和核酸的去磷酸化。

Physical properties
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

牛肠碱性磷酸酶为二聚膜衍生糖蛋白。至少存在三种同工​酶，每个单体通常拥有两个 N-连接多糖和一个或多个 O-连接多糖。2 该酶需要锌以及镁或钙二价离子才能显示活性。
性质
Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase, Alkaline Phosphatase Enzymes, Analytical and Industrial Enzymes,
Application Index, Bovine Alkaline Phosphatase, Enzyme Class Index, Immunolabeling, 生化试剂, 酶、抑制剂和底物
更多...
product line BioUltra
form buffered aqueous glycerol solution
mol wt dimer mol wt ~160 kDa
storage temp. 2-8°C

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