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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
GATA Binding Protein 2 (GATA2) (N-Term) 抗体
- 规格:
0.1ml/0.2ml
GATA Binding Protein 2 (GATA2)
别名 GATA2, Gata2, DCML, MONOMAC, NFE1B, xgata-2, xgata2, GATA-2, NF-E1b, Gata-2
抗原表位
其他选择 N-Term
适用
其他选择 小鼠, 大鼠, 犬, 牛, 猪, 非洲爪蟾, 人, 小鸡
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 免疫组织化学(IHC), Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品编号 yb182422
产品细节 GATA Binding Protein 2 (GATA2) (N-Term) 抗体
免疫原 Synthetic peptide directed towards the N terminal of human GATA2
序列 PYYANPAHAR ARVSYSPAHA RLTGGQMCRP HLLHSPGLPW LDGGKAALSA
预测反应 Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, African clawed frog : 90 %, Chicken : 90 %
产品特性 This is a rabbit polyclonal antibody against GATA2. It was validated on Western Blot and immunohistochemistry.
纯化方法 Protein A purified
目标详细情况GATA Binding Protein 2 (GATA2) (N-Term) 抗体
别名 GATA2
背景 The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.
分子量 50 kDa
基因ID 2624
NCBI登录号 NM_032638, NP_116027
UniProt P23769
研究领域 Cardiogenesis, Lineage Markers, Transcription Factors
使用细节GATA Binding Protein 2 (GATA2) (N-Term) 抗体
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 480 AA
限制 仅限研究用
贮存及处理GATA Binding Protein 2 (GATA2) (N-Term) 抗体
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
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文献和实验-terminally to the transcription factor of interest is very efficiently biotinylated by BirA coexpressed in the same cells, as was demonstrated by the tagging of the essential hematopoietic transcription factor GATA-1. The direct binding to streptavidin
的突变(IDH1,IDH2和RAS)并没有在AEL中出现。 GATA2是一个和造血功能相关的锌指转录因子,在该研究中共有13个AEL出现突变。分析显示,这些突变影响7个不同的氨基酸区域。其中,10个病人的突变位于N-terminal ZF1,3个病人的突变位于转录激活区域(Q20H),12个病人的突变为错义突变,其中又有9个位于DNA-binding N-terminal zinc-finger区域。此外,为了探究GATA2与血液病的关系,研究人员对253个non-AEL AML,40
有关Production of soluble mammalian proteins in Escherichia coli的经验的一篇好文章
protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors,CD44), kinases (EGFR-cytoplasmic domain, CDK2 and 4), proteases (MMP1, CASP2), signal transduction proteins (GRB2, RAF1, HRAS) and transcription factors (GATA2, Fli1, Trp
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