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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
RNA Polymerase II CTD (C-Term) 抗体
- 规格:
0.1ml/0.2ml
RNA Polymerase II CTD
别名
5, 8WG16, CG1554, CTD, Dmel\\CG1554, H5, II, L5, POL, Pol II, Pol II CTD, Pol II Ser5P, Pol II0[ser2], Pol II0[ser5], Pol II[ser2], Pol IIa, Pol IIo, Pol IIo[Ser2], Pol IIo[ser2], Pol IIo[ser5], Pol-I ... show more
抗原表位
其他选择 C-Term
适用
其他选择 小鼠, 人, Yeast (Saccharomyces cerevisiae), Arabidopsis thaliana, Caenorhabditis elegans (C. elegans), Fruit Fly (Drosophila melanogaster), Schizosaccharomyces pombe, Green Monkey (Chlorocebus sabaeus)
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (4H8)
RNA Polymerase II CTD (C-Term) 抗体应用范围
其他选择 Chromatin Immunoprecipitation (ChIP), 酶联免疫吸附测定(ELISA), 流式细胞分析技术(FACS), 免疫细胞化学(ICC), 免疫萤光(IF), 免疫沉淀(IP), Western Blotting (WB)
Pubmed 4个引用可参考
规格 50 μL
发货至 中国 (更改)
发货时间 25至30个工作日
产品编号 yb108592
产品细节
免疫原 Synthetic peptide: YSPTSpPS (Human). The sequence is repeated multiple times in the C-terminal domain of RNA polymerase II
克隆位点 4H8
亚型 IgG1
特异性 Recognises the C-terminal repeat of the largest subunit of RNA polymerase II. It recognises both unphosphorylated and phosphorylated forms.
产品特性 Myeloma fusion partners:
Sp2/0 Ag.14
纯化方法 Protein A purified
质量等级 ChIP Grade
目标详细情况
RNA Polymerase II CTD (C-Term) 抗体背景 Entrez Gene: 177190 Caenorhabditis elegans, Entrez Gene: 32100 Fruit fly (Drosophila melanogaster), Entrez Gene: 5430 Human, Entrez Gene: 20020 Mouse, Entrez Gene: 851415 Saccharomyces cerevisiae
Unigene: 2925 Fruit fly (Drosophila melanogaster) Unigene: 270017 Human Unigene: 16533 Mouse
Synonyms:
DNA directed RNA polymerase II A antibody,DNA-directed RNA polymerase II largest subunitantibody,DNA-directed RNA polymerase II subunit A antibody,DNA-directed RNA polymerase II subunit RPB1antibody,DNA-directed RNA polymerase III largest subunit antibody,Polr2a antibody,RNA pol II CTD antibody,RNApolymerase II subunit B1 antibody,RNA-directed RNA polymerase II subunit RPB1 antibody,RPB1 antibody,RPB1_HUMANantibody
基因ID 177190, 32100, 5430
OMIM 180660
UniProt P08775, P04052, P18616, P16356, P24928
研究领域 DNA/RNA, Cell Cycle, Signaling
使用细节
应用备注 Optimal antibody dilution should be determined by titration
限制 仅限研究用
贮存及处理
状态 Liquid
浓度 0.8 mg/mL
缓冲液 PBS, 0.1 % sodium azide
储存液 Sodium azide
RNA Polymerase II CTD (C-Term) 抗体注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件 4 °C
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文献和实验An RNA Polymerase II In Vitro Transcription System
A great deal of attention in recent years has been focused on the mechanisms governing transcription. Repression and/or activation of specific genes, or sets of genes, represents a key regulatory step in such diverse processes as cell growth
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
polymerase II (Pol II) chromatin immunoprecipitation (ChIP) is a simpler alternative to determine the transcription rate of genes. Moreover, this approach provides more information about the transcriptional regulation of a gene than nuclear run-on. The power
RNA polymerase II (Pol II) plays a crucial role in eukaryotic biology since it is necessary for the expression of all protein-coding genes as well as most microRNAs and several small nuclear RNAs. Pol II is specifically recruited to core
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