CD34 Molecule (CD34) 抗体 (FITC)抗原
CD34 Molecule (CD34)
别名 AU040960
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (MEC14-7)
标记
其他选择 FITC
CD34 Molecule (CD34) 抗体 (FITC)应用范围
其他选择 流式细胞分析技术(FACS)
Pubmed 找到1个引用
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb119259
产品细节
亚型 IgG2a
特异性 This antibody recognises the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. It recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
交叉反应 (详细) Species reactivity (tested):Mouse.
纯化方法 Affinity Chromatography on Protein G.
目标详细情况
别名 CD34
CD34 Molecule (CD34) 抗体 (FITC)背景 The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
基因ID 12490
NCBI登录号 NP_598415
UniProt Q64314
研究领域 Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
使用细节
应用备注 Flow cytometry: use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
浓度 0.1 mg/mL
缓冲液 PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD34 Molecule (CD34) 抗体 (FITC)注意事项 Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.