- ¥2506
- LONZA
- 12-769E
- 2025年08月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
研卉生物
- 英文名：
ProFreeze-CDM, NAO, 2x Freeze Media, 100 ml
- 规格：
瓶
ProFreeze-CDM, NAO, 2x Freeze Media, 100 ml 12-769E
Product Overview:
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS). However, it is used to greatest advantage with cells cultured in a serum-free and animal component-free environment. This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.
ProFreeze™ requires the addition of 15% reagent or spectrophotometric grade DMSO be added at the time of use. One bottle will make 117.6 ml of complete 2X concentrated freezing medium after the addition of 17.6 ml DMSO. For best results, keep on ice during use.
Storage conditions: 2ºC - 8ºC
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文献和实验0.1% Pluronic F-68 1L Permexcis® Chemically Defined Virus Prod Lonza BE02-039Q PERMEXCIS(TM) Virus production medium, a PER.C6® medium 1L bottle 冻存液 Lonza 12-769E ProFreeze-CDM, NAO, 2x Freeze Media, 100ml LONZA龙沙授权代理商及代理区域--北京毕特博
Phenol/Freeze RNA Prep Schmitt et al. (1990) NAR 18, 3091-3092. DEPC is used to rid solutions of RNases. DEPC is very toxic and should be used only in the hood. Stuff you need: (DEPC treatment means: add DEPC to 0.1%, mix O/N
Preparation of NeoR Murine Embryonic Fibroblasts
with MEF media. Spin cells in a centrifuge at 1000 RPM for 5 minutes at 10oC. Resuspend the pellet in 5.0ml MEF media, pipette up and down to break the pellet into single cells, add 0.5ml MEF cells in each of 10 Nunc cryotubes, add 0.5ml 2X MEF Freezing
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