Cell Signaling CST 一抗 2946P p2

Use of an immobilized monoclonal antibody to examine integrin 51 signaling independent of cell spreading

-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21CIP1 and p27KIP1 , while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin α5β1 induces integrin signaling regulating

WB实战指南（四）之 难点：抗体孵育

文献的话很容易找到这家公司的。可惜，在国内代理商不可能提供相应的服务，因此购买他们的商品存在不小的风险。所以，通常我更推荐sigma和Abcom、R&D等。诸如Sigma公司销售的Actin，cat. A5316，clone AC-74，Mab更是作为新手练习专用的抗体；它的背景很低，效价很高，可以1：30,000稀释（是普通一抗效价的30倍）。此外，尽管cell signaling是做磷酸化抗体起家的公司，但它们的抗体的质量总体感觉也不好，所以有其它公司可以选择时，我们会刻意避免购买cell

TRAF‐Mediated TNFR‐Family Signaling

. Cell 80:389‐399.    Muzio, M., Ni, J., Feng, P., and Dixit, V.M. 1997. IRAK (Pelle) family member IRAK‐2 and MyD88 as proximal mediators of IL‐1 signaling. Science 278:1612‐1615